Skip to main content
Addgene

mRIP3 GFP wt
(Plasmid #41382)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RIP3
  • Alt name
    RIPK3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1545
  • GenBank ID
    NM_019955
  • Entrez Gene
    Ripk3 (a.k.a. 2610528K09Rik, Rip3)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRIP3 GFP wt was a gift from Francis Chan (Addgene plasmid # 41382 ; http://n2t.net/addgene:41382 ; RRID:Addgene_41382)

  • For your References section:

    Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cho YS, Challa S, Moquin D, Genga R, Ray TD, Guildford M, Chan FK. Cell. 2009 Jun 12;137(6):1112-23. 10.1016/j.cell.2009.05.037 PubMed 19524513
Related items:
Commonly requested with: