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Addgene

UAS-DExt (ttv)
(Plasmid #41180)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dext
  • Alt name
    ttv
  • Alt name
    tout-velu
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    ttv (a.k.a. Dmel_CG10117, CG10117, DEXT1, Dmel\CG10117, EXT1, Ext1, P1.15, Ttv, dExt1, l(2)00681, l(2)05282, l(2)SH0631, l(2)SH2 0631, l(2)k00115, l(2)k03617)
  • Promoter Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS-DExt (ttv) was a gift from Norbert Perrimon (Addgene plasmid # 41180 ; http://n2t.net/addgene:41180 ; RRID:Addgene_41180)

  • For your References section:

    Tout-velu is a Drosophila homologue of the putative tumour suppressor EXT-1 and is needed for Hh diffusion. Bellaiche Y, The I, Perrimon N. Nature. 1998 Jul 2;394(6688):85-8. 10.1038/27932 PubMed 9665133
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