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Addgene

hs-FL-Kek1-myc2
(Plasmid #41170)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR-hs
  • Backbone size w/o insert (bp) 8780
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kek1
  • Alt name
    Kek1 full-length
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    kek1 (a.k.a. Dmel_CG12283, BEST:GM02380, CG12283, CT18186, Dmel\CG12283, Dmkek1, GM02380, KEK1, Kek, Kek-1, Kek1, NB1, SD01674, kek, kek-1, kekon, l(2)01433, n(2)k07332, uns)
  • Promoter Hsp70
  • Tag / Fusion Protein
    • 2X myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hs-FL-Kek1-myc2 was a gift from Norbert Perrimon (Addgene plasmid # 41170 ; http://n2t.net/addgene:41170 ; RRID:Addgene_41170)

  • For your References section:

    The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis. Ghiglione C, Carraway KL 3rd, Amundadottir LT, Boswell RE, Perrimon N, Duffy JB. Cell. 1999 Mar 19;96(6):847-56. 10.1016/S0092-8674(00)80594-2 PubMed 10102272
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