pRcCMV Rootletin (Nigg pFL6(CW501))
(Plasmid #41167)

• Depositing Lab: Erich Nigg
• Publication: Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. Epub 2005 Oct 3.

Backbone

• Vector backbone: pCJW206 (modified pEGFP-C1)
• Backbone manufacturer: Modified from Clontech
• Backbone size w/o insert (bp): 4110
• Total vector size (bp): 10100
• Modifications to backbone: FseI- and NotI-site-containing linker (AAT TCG GCC GGC CTA GCG GCC GCT GCA) inserted between EcoRI and PstI sites. Myc-tag-encoding fragment (aag ctt gct tac att tgc ttc tga cac aac tgt gtt cac tag caa cct caa aca gac acc atg gag cag aag ctg atc tcc gag gag gac ctg aac atg aat tc) inserted between HindIII and EcoRI sites. GFP-encoding sequence removed by excision with BspEI and AgeI and religation
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rootletin
• Alt name: CROCC
• Alt name: ciliary rootlet coiled-coil, rootletin
• Species: H. sapiens (human)
• Insert Size (bp): 6000
• GenBank ID:
• Entrez Gene: CROCC (a.k.a. ROLT, TAX1BP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Partial cDNA clone KIAA0445 from Kazusa DNA Research Institute and IMAGE clone 6150861 from Deutsches Ressourcenzentrum fuer Genomforschung GmbH
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The partial cDNA clones KIAA0445 (Kazusa DNA Research Institute) and IMAGE clone 6150861 (Deutsches Ressourcenzentrum fuer Genomforschung GmbH) were fused, and the complete coding sequence for rootletin was cloned into pBluescript II SK- (Addgene plasmid #41168) and subsequently subcloned into pEGFP-C1 (CLONTECH Laboratories, Inc.) (Addgene plasmid #41166). pRcCMV Rootletin (Nigg pFL6(CW501)) (Addgene plasmid #41167), encoding myc-rootletin, was prepared by replacing the GFP tag. All constructs were confirmed by sequencing.