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Addgene

pRcCMV myc-Plk1 T210D (Nigg LA183)
(Plasmid #41158)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5527
  • Total vector size (bp) 7746
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plk1
  • Alt name
    STPK13
  • Alt name
    polo-like kinase 1
  • Alt name
    serine/threonine-protein kinase 13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2219
  • Mutation
    T210D
  • GenBank ID
    NM_005030.3 X73458.1
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRcCMV myc-Plk1 T210D (Nigg LA183) was a gift from Erich Nigg (Addgene plasmid # 41158 ; http://n2t.net/addgene:41158 ; RRID:Addgene_41158)

  • For your References section:

    Polo-like kinase-1 is a target of the DNA damage checkpoint. Smits VA, Klompmaker R, Arnaud L, Rijksen G, Nigg EA, Medema RH. Nat Cell Biol. 2000 Sep;2(9):672-6. 10.1038/35023629 PubMed 10980711
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