pcDNA3 Lats1 (Nigg HS189)
(Plasmid #41156)

• Depositing Lab: Erich Nigg
• Publication: Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

Backbone

• Vector backbone: pcDNA3.1/3x myc-C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lats1
• Alt name: LATS, large tumor suppressor, homolog 1
• Alt name: WARTS
• Alt name: wts
• Species: H. sapiens (human)
• Insert Size (bp): 3300
• GenBank ID: NM_001270519.1 NM_004690.3;
• Entrez Gene: LATS1 (a.k.a. WARTS, wts)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xMyc (N terminal on backbone)
  • PreScission Site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI/BglII (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human LATS1 cDNA from Hideyuki Saya.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human LATS1 cDNA, a kind gift from Hideyuki Saya, was cloned into pcDNA3.1/3x myc-C encoding an amino-terminal 3 x myc tag and verified by sequencing.