pcDNA3 CAP350 (Nigg XY97)
(Plasmid #41146)

• Depositing Lab: Erich Nigg
• Publication: Yan et al Mol Biol Cell. 2006 Feb;17(2):634-44. Epub 2005 Nov 28.

Backbone

• Vector backbone: pcDNA3.1/3x myc-A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 15000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAP350
• Alt name: CEP350
• Alt name: GM133
• Alt name: centrosomal protein 350kDa
• Species: H. sapiens (human)
• Insert Size (bp): 9500
• GenBank ID: NM_014810.4
• Entrez Gene: CEP350 (a.k.a. CAP350, GM133)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xMyc (N terminal on backbone)
  • PreScission Site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAP350 cDNA clone fragments from Deutsches Ressourcenzentrum fur Genomforschung (Berlin), and Dr. S. Sugano (Institute of Medical Science, University of Tokyo, Japan).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3
' region of a CAP350 cDNA clone (KIAA0480) was obtained from Deutsches Ressourcenzentrum fur Genomforschung (Berlin), and the overlapping KIAA2443 cDNA was kindly provided by Dr. S. Sugano (Institute of Medical Science, University of Tokyo, Japan). The missing 5
' region was amplified by RT-PCR from HeLa S3 total RNA, using sequence information from GenBank (Accession No. AF287356). cDNAs were ligated to yield full-length CAP350 cDNA, confirmed by DNA sequencing, and subcloned into pCRII-TOPO (Invitrogen, Carlsbad, CA). N-terminally myc-tagged CAP350 was constructed by subcloning into mammalian pCMV expression vector pcDNA3.1/3x myc-A by standard procedures.