pSPORT-RREB1
(Plasmid #41145)

• Depositing Lab: Joshua Mendell
• Publication: Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2012.266.

Backbone

• Vector backbone: pSPORT6.1
• Backbone size w/o insert (bp): 4149
• Total vector size (bp): 9404
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RREB-1
• Alt name: RREB1
• Alt name: hZIP
• Species: H. sapiens (human)
• Insert Size (bp): 5255
• GenBank ID: NP_001003698.1
• Entrez Gene: RREB1 (a.k.a. FINB, HNT, LZ321, RREB-1, Zep-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains the G195R natural variant in RREB1