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Addgene

siCheck2-4xmiR307_21nt-seedonly
(Plasmid #41101)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    siCheck2
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 6200
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4xmiR307_21nt-seedonly
  • Species
    Synthetic
  • Insert Size (bp)
    92

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer ATGAAATGGGTAAGTACATCAAGAGCTTCG
  • 3′ sequencing primer TCCTCACACAAAAAACCAACACACAGATGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    siCheck2-4xmiR307_21nt-seedonly was a gift from Phillip Zamore (Addgene plasmid # 41101 ; http://n2t.net/addgene:41101 ; RRID:Addgene_41101)

  • For your References section:

    Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD. Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027. 10.1016/j.cell.2012.09.027 PubMed 23063653
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