pcold-6xHis-HRV3Csite-HsPACT
(Plasmid #41097)

• Depositing Lab: Phillip Zamore
• Publication: Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027.

Backbone

• Vector backbone: pcold1
• Backbone manufacturer: Takara
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 6900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PACT
• Alt name: interferon-inducible double stranded RNA-dependent protein kinase activator A
• Species: H. sapiens (human)
• Insert Size (bp): 942
• Entrez Gene: PRKRA (a.k.a. DYT16, HSD14, PACT, RAX)
• Tag / Fusion Protein:
  • 6xHis-HRV3Csite (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GTAATACACCATGAATCACAAAGTG
• 3′ sequencing primer: AATAAAAAAATCCCCGCCAAATGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry