Skip to main content
Addgene

pcold-6xHis-HRV3Csite-DmLoqsPB
(Plasmid #41094)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcold1
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Loquacious-PB
  • Alt name
    Loqs-PB
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1398
  • Entrez Gene
    loqs (a.k.a. Dmel_CG6866, CG6866, Dmel\CG6866, LOQS, Loqs, Loqs-PD, LqPD, R3D1, R3D1-L, R3D1-S, TRBP, cg6866, dRax, loq, r3d1)
  • Tag / Fusion Protein
    • 6xHis-HRV3Csite (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GTAATACACCATGAATCACAAAGTG
  • 3′ sequencing primer AATAAAAAAATCCCCGCCAAATGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcold-6xHis-HRV3Csite-DmLoqsPB was a gift from Phillip Zamore (Addgene plasmid # 41094 ; http://n2t.net/addgene:41094 ; RRID:Addgene_41094)

  • For your References section:

    Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD. Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027. 10.1016/j.cell.2012.09.027 PubMed 23063653
Related items:
Commonly requested with: