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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHM-GWA
- Backbone size w/o insert (bp) 8236
- Total vector size (bp) 7344
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCsy4
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Alt namePa14_33300
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)564
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MutationHis29Ala mmutation to abolish ribonuclease activity
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GenBank ID115585590 ABJ11605
- Promoter T7
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Tag
/ Fusion Protein
- His6-MBP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MBP forward
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information on Doudna Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/doudna/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHMGWA-Pa14Csy4H29A was a gift from Jennifer Doudna (Addgene plasmid # 41092 ; http://n2t.net/addgene:41092 ; RRID:Addgene_41092) -
For your References section:
Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA. Science. 2010 Sep 10;329(5997):1355-8. 10.1126/science.1192272 PubMed 20829488