pEGFP-N1-RACK1
(Plasmid #41088)

• Depositing Lab: Anna Huttenlocher
• Publication: Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5669
• Modifications to backbone: PCR-amplified insert was blunt-end ligated into pPCR-Script AMP vector, removed by digestion with HindIII and KpnI, and ligated into pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Receptor for activated C kinase
• Alt name: RACK1
• Alt name: GNB2L1
• Species: H. sapiens (human)
• Insert Size (bp): 954
• GenBank ID: NM_006098.4
• Entrez Gene: RACK1 (a.k.a. GNB2L1, Gnb2-rs1, H12.3, HLC-7, PIG21)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP_C_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pcDNA3.1 RACK1 was recieved from T.E. O'Toole at Scripps Research Institute then cloned into N1-pEGFP
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry