pCI-6.22
(Plasmid #41087)

• Depositing Lab: James Inglese
• Publication: Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.

Backbone

• Vector backbone: pGL3-Enhancer
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5064
• Total vector size (bp): 6200
• Modifications to backbone: The STOP codon of FLuc was removed and replaced by an in-frame EcoRI site.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSG-P2A-RLuc
• Insert Size (bp): 1014
• Promoter: Promoterless

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: EBV-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This pCI-6.22 is a promoterless version of FLuc-P2A-RLuc coincident reporter plasmid. It contains a MCS in the 5' region for people to study their favorite promoter/response element. Please check the map of pGL3-Enhancer (Promega) for the infomation about the MCS.

Please note that the E304V point mutation found in the Addgene sequence does not affect the activity of this plasmid.