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Addgene

pBabepuroBAF180
(Plasmid #41078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Weinberg lab (Addgene Plasmid #1764)
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 10130
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To avoid new spontaneous mutations, we try to harvest the bacteria for DNA in the late log or early saturation phase of growth (after having selected individual colonies).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAF180
  • Alt name
    PBRM1
  • Alt name
    PB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4961
  • Mutation
    3 silent mutations: 3595 a to g; 4981 a to t; 5875 a to g
  • GenBank ID
    NM_018313.4
  • Entrez Gene
    PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabepuroBAF180 was a gift from Ramon Parsons (Addgene plasmid # 41078 ; http://n2t.net/addgene:41078 ; RRID:Addgene_41078)

  • For your References section:

    BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer. Xia W, Nagase S, Montia AG, Kalachikov SM, Keniry M, Su T, Memeo L, Hibshoosh H, Parsons R. Cancer Res. 2008 Mar 15;68(6):1667-74. 10.1158/0008-5472.CAN-07-5276 PubMed 18339845
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