Skip to main content
Addgene

pQCXIP-HA.Tetherin
(Plasmid #41069)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7162
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetherin
  • Alt name
    BST-2
  • Alt name
    bone marrow stromal cell antigen 2
  • Alt name
    CD317
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    550
  • GenBank ID
    NM_004335.2 NP_004326.1
  • Entrez Gene
    BST2 (a.k.a. CD317, HM1.24, TETHERIN)
  • Promoter CMV/MSV
  • Tags / Fusion Proteins
    • IRES Puro (C terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a viral-delivery-compatible HA.Tetherin expression construct. It was generated by cloning of tetherin from plasmid pCMV-HA.Tetherin (Addgene plasmid #41068) into AgeI and PacI restriction sites of pQCXIP (Clontech).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP-HA.Tetherin was a gift from Paul Spearman (Addgene plasmid # 41069 ; http://n2t.net/addgene:41069 ; RRID:Addgene_41069)

  • For your References section:

    Immunoelectron microscopic evidence for Tetherin/BST2 as the physical bridge between HIV-1 virions and the plasma membrane. Hammonds J, Wang JJ, Yi H, Spearman P. PLoS Pathog. 2010 Feb 5;6(2):e1000749. 10.1371/journal.ppat.1000749 PubMed 20140192
Related items:
Commonly requested with: