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Purpose(Empty Backbone)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTKS/CS
- Backbone size (bp) 3251
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)BW25113
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Growth instructionsHigh expression of tetA makes the host strain growth rate extremely slow. Please allow sufficient time for growth.
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTKS/CS was a gift from Edward Cox & Thomas Kuhlman (Addgene plasmid # 41063) -
For your References section:
Site-specific chromosomal integration of large synthetic constructs. Kuhlman TE, Cox EC. Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4. 10.1093/nar/gkp1193 PubMed 20047970