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Addgene

Foxn1-pBSII SK
(Plasmid #41032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    in situ probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Foxn1 in situ probe
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Foxn1 (a.k.a. D11Bhm185e, Fkh19, HFH-11, Hfh11, Whn, nu, nude)
  • Promoter T7 sense; T3 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For Foxn1 in situ antisense probe, linearize with AccIII and transcribe with T3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Foxn1-pBSII SK was a gift from Nancy Manley (Addgene plasmid # 41032 ; http://n2t.net/addgene:41032 ; RRID:Addgene_41032)

  • For your References section:

    Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains in the developing third pharyngeal pouch. Gordon J, Bennett AR, Blackburn CC, Manley NR. Mech Dev. 2001 May;103(1-2):141-3. 10.1016/S0925-4773(01)00333-1 PubMed 11335122
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