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Addgene

Gcm2TEASY
(Plasmid #41031)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3015
  • Vector type
    in situ probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gcm2 in situ probe
  • Species
    M. musculus (mouse)
  • Mutation
    contains nucleotides 584-1510 of GenBank accession number AF081556
  • GenBank ID
    AF081556
  • Entrez Gene
    Gcm2 (a.k.a. Gcm-rs1, Gcm1, Gcm1-rs2)
  • Promoter T7 sense; SP6 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For Gcm2 in situ antisense probe, linearize with NcoI and transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gcm2TEASY was a gift from Nancy Manley (Addgene plasmid # 41031 ; http://n2t.net/addgene:41031 ; RRID:Addgene_41031)

  • For your References section:

    Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains in the developing third pharyngeal pouch. Gordon J, Bennett AR, Blackburn CC, Manley NR. Mech Dev. 2001 May;103(1-2):141-3. 10.1016/S0925-4773(01)00333-1 PubMed 11335122
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