pFA6a-natMX-tetO-Pcyc1-3xFLAG
(Plasmid
#41021)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 41021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFA6a
-
Vector typeN-terminal tagging/promoter replacement
-
Selectable markersNourseothricin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nametetO7-Pcyc1-3xFLAG
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few point mutations in the drug resistance gene. These mutations are derived from the original drug resistant plasmids we obtained and do not alter the drug resistant property of the gene or the function of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a-natMX-tetO-Pcyc1-3xFLAG was a gift from Michael Nick Boddy (Addgene plasmid # 41021 ; http://n2t.net/addgene:41021 ; RRID:Addgene_41021) -
For your References section:
A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe. Zilio N, Wehrkamp-Richter S, Boddy MN. Yeast. 2012 Aug 29. doi: 10.1002/yea.2920. 10.1002/yea.2920 PubMed 22968950