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Addgene

pCAGIG-6xHIS.Mm.RPE65-eGFP
(Plasmid #41019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGIG
  • Backbone manufacturer
    Connie Cepko (Addgene plasmid #11159)
  • Backbone size w/o insert (bp) 6135
  • Vector type
    Mammalian Expression
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPE65
  • Alt name
    retinal pigment epithelium 65
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rpe65 (a.k.a. 65kDa, A930029L06Rik, LCA2, Mord1, RP20, rd12)
  • Promoter CAG
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer pCAGGS5
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses EGFP as a reporter gene from IRES as part of the bicistronic transcript

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGIG-6xHIS.Mm.RPE65-eGFP was a gift from John Nickerson (Addgene plasmid # 41019 ; http://n2t.net/addgene:41019 ; RRID:Addgene_41019)
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