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PurposePCR template for en passant (two-step Red-mediated) mutagenesis
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Total vector size (bp) 6288
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKana_I-SceI
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPkan-S was a gift from Nikolaus Osterrieder (Addgene plasmid # 41017 ; http://n2t.net/addgene:41017 ; RRID:Addgene_41017) -
For your References section:
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. Tischer BK, von Einem J, Kaufer B, Osterrieder N. Biotechniques. 2006 Feb;40(2):191-7. 10.2144/000112096 PubMed 16526409
Map uploaded by the depositor.
