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Addgene

pCS2 YFP-Smo-Weyer'sAla5
(Plasmid #41016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4078
  • Total vector size (bp) 7329
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    smoothened homolog
  • Alt name
    Smo
  • Species
    M. musculus (mouse)
  • Mutation
    YFP inserted after Smo signal sequence. Transferred last 44 amino acids of dominant negative mouse EVC2 alanine mutant to C-terminus.
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
  • Tags / Fusion Proteins
    • EVC2 ala5 (aa 1177-1220 with amino acids 1185-1188 changed to Alanine) (C terminal on insert)
    • YFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 YFP-Smo-Weyer'sAla5 was a gift from Rajat Rohatgi (Addgene plasmid # 41016 ; http://n2t.net/addgene:41016 ; RRID:Addgene_41016)

  • For your References section:

    A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary Cilia. Dorn KV, Hughes CE, Rohatgi R. Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 10.1016/j.devcel.2012.07.004. 10.1016/j.devcel.2012.07.004 PubMed 22981989
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