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Addgene

hSyn1-FLEX-hChR2-tdtomato
(Plasmid #41015)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV (Adeno-associated Virus)
  • Backbone size w/o insert (bp) 4971
  • Total vector size (bp) 7344
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Alt name
    COP4
  • Alt name
    CHLREDRAFT_182032
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
    2373
  • Mutation
    codon-optimized (human)
  • GenBank ID
    COP4 (CHLREDRAFT_182032) XP_001701725.1
  • Promoter synapsin
  • Tag / Fusion Protein
    • tdtomato (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer FLEX-R (ctcttcgagggacATAACTTCG)
  • 3′ sequencing primer tdTomato-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn1-FLEX-hChR2-tdtomato was a gift from Karel Svoboda (Addgene plasmid # 41015 ; http://n2t.net/addgene:41015 ; RRID:Addgene_41015)
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