pCS2 YFP-Smo-Weyer's
(Plasmid
#41014)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4078
- Total vector size (bp) 7329
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesmoothened homolog
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Alt nameSmo
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SpeciesM. musculus (mouse)
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MutationYFP inserted after Smo signal sequence. Transferred last 44 amino acids of mouse EVC2 to C-terminus.
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Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
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Tags
/ Fusion Proteins
- EVC2 (aa 1177-1220) (C terminal on insert)
- YFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 YFP-Smo-Weyer's was a gift from Rajat Rohatgi (Addgene plasmid # 41014 ; http://n2t.net/addgene:41014 ; RRID:Addgene_41014) -
For your References section:
A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary Cilia. Dorn KV, Hughes CE, Rohatgi R. Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 10.1016/j.devcel.2012.07.004. 10.1016/j.devcel.2012.07.004 PubMed 22981989