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Addgene

pEF5/FRT-DEST EVC2ala7-Flag
(Plasmid #41010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF5/FRT/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 11200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ellis van Creveld syndrome 2
  • Alt name
    EVC2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3659
  • Mutation
    changed amino acids 1189-1192 to Alanine
  • Entrez Gene
    EVC2 (a.k.a. LBN, WAD)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5/FRT-DEST EVC2ala7-Flag was a gift from Rajat Rohatgi (Addgene plasmid # 41010 ; http://n2t.net/addgene:41010 ; RRID:Addgene_41010)

  • For your References section:

    A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary Cilia. Dorn KV, Hughes CE, Rohatgi R. Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 10.1016/j.devcel.2012.07.004. 10.1016/j.devcel.2012.07.004 PubMed 22981989
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