pEF5/FRT-DEST EVC2-TEV-YFP-Flag
(Plasmid
#41004)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF5/FRT/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7500
- Total vector size (bp) 12000
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEllis van Creveld syndrome 2
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Alt nameEVC2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3659
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Entrez GeneEVC2 (a.k.a. LBN, WAD)
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Tag
/ Fusion Protein
- TEV-YFP-Flag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF5/FRT-DEST EVC2-TEV-YFP-Flag was a gift from Rajat Rohatgi (Addgene plasmid # 41004 ; http://n2t.net/addgene:41004 ; RRID:Addgene_41004) -
For your References section:
A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary Cilia. Dorn KV, Hughes CE, Rohatgi R. Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 10.1016/j.devcel.2012.07.004. 10.1016/j.devcel.2012.07.004 PubMed 22981989