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Addgene

pCI-6.20
(Plasmid #41002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Total vector size (bp) 6264
  • Modifications to backbone
    The STOP codon of FLuc was removed and replaced by an in-frame EcoRI site.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSG-P2A-RLuc
  • Insert Size (bp)
    1014
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer EBV-Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the E304V point mutation found in the Addgene sequence does not affect the activity of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-6.20 was a gift from James Inglese (Addgene plasmid # 41002 ; http://n2t.net/addgene:41002 ; RRID:Addgene_41002)

  • For your References section:

    A coincidence reporter-gene system for high-throughput screening. Cheng KC, Inglese J. Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170. 10.1038/nmeth.2170 PubMed 23018994
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