pcDNA-p160MBP
(Plasmid #41)

• Depositing Lab: Bruce Spiegelman
• Publication: Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26.

Backbone

• Vector backbone: pcDNA3.1 myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p160 myb binding protein
• Alt name: Mybbp1a
• Alt name: p160MBP
• Species: M. musculus (mouse)
• Insert Size (bp): 4000
• GenBank ID: BC052889
• Entrez Gene: Mybbp1a (a.k.a. AL024407, AU019902, P160, p160MBP, p67MBP)
• Tag / Fusion Protein:
  • myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tom Gonda
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a K935R mutation in this insert. The mutation is not known to affect p160MBP function.