pcDNA5/FRT/TO-Venus-Flag (865)
(Plasmid #40998)

• Purpose: (Empty Backbone)
• Depositing Lab: Jonathon Pines
• Publication: Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 10.1038/emboj.2012.335. Epub 2013 Jan 4.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Modifications to backbone: Venus YFP-3xFlag inserted via BstXI-XhoI sites
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Hygromycin
• Tags / Fusion Proteins:
  • Venus YFP (C terminal on insert)
  • 3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: EGFP-N, BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Venus YFP-3xFlag was inserted into this vector via BstXI-XhoI sites.

This vector can be used to express proteins fused to Venus YFP with a C-terminal 3xFlag tag.

The MCS consists of PmeI (non-unique), AflII, HindIII, Asp718I, KpnI, BamHI, and BstXI upstream of Venus. Additionally, the following sites are present after the Venus-3xFlag sequence: XhoI, Eco0109I, ApaI, PmeI (non-unique), and BclI.