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pT7-Venus-cdc25C5
(Plasmid #40969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-Venus-C1 (785)
  • Backbone manufacturer
    Jonathan Pines Lab (Gurdon Institute, University of Cambridge, UK)
  • Modifications to backbone
    From pEYFP-C1: EYFP was replaced with Venus using AgeI and BsrGI restriction sites, and the CMV promoter was replaced with a T7 promoter using NdeI and NheI sites. Note that the SacI and HindIII sites in the MCS are no longer unique.
  • Vector type
    In Vitro Transcription
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc25C5
  • Alt name
    cdc25C
  • Alt name
    Cell Division Cycle 25 Homolog C
  • Alt name
    M-phase inducer phosphatase 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1215
  • GenBank ID
    NM_022809.2 NP_073720.1
  • Entrez Gene
    CDC25C (a.k.a. CDC25, PPP1R60)
  • Promoter T7
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-Venus-cdc25C5 was a gift from Olivier Gavet (Addgene plasmid # 40969 ; http://n2t.net/addgene:40969 ; RRID:Addgene_40969)
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