pT7-Venus-cdc25C1
(Plasmid #40968)

• Depositing Lab: Olivier Gavet
• Publication: Olivier Gavet lab cdc25C splice variant plasmids (unpublished)

Backbone

• Vector backbone: pT7-Venus-C1 (785)
• Backbone manufacturer: Jonathan Pines Lab (Gurdon Institute, University of Cambridge, UK)
• Modifications to backbone: From pEYFP-C1: EYFP was replaced with Venus using AgeI and BsrGI restriction sites, and the CMV promoter was replaced with a T7 promoter using NdeI and NheI sites. Note that the SacI and HindIII sites in the MCS are no longer unique.
• Vector type: In Vitro Transcription
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cdc25C5
• Alt name: cdc25C
• Alt name: Cell Division Cycle 25 Homolog C
• Alt name: M-phase inducer phosphatase 3
• Species: H. sapiens (human)
• Insert Size (bp): 1434
• GenBank ID: NM_001790.3 NP_001781.2
• Entrez Gene: CDC25C (a.k.a. CDC25, PPP1R60)
• Promoter: T7
• Tag / Fusion Protein:
  • Venus (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry