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Addgene

EGFP-bSV-831-1281
(Plasmid #40962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 6081
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    supervillin
  • Alt name
    SVIL
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1350
  • Mutation
    deleted amino acids 1-830 and 1282-1792
  • Entrez Gene
    SVIL
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer EGFP
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-bSV-831-1281 was a gift from Elizabeth Luna (Addgene plasmid # 40962 ; http://n2t.net/addgene:40962 ; RRID:Addgene_40962)

  • For your References section:

    Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals. Wulfkuhle JD, Donina IE, Stark NH, Pope RK, Pestonjamasp KN, Niswonger ML, Luna EJ. J Cell Sci. 1999 Jul:112 (Pt 13):2125-36. doi: 10.1242/jcs.112.13.2125 10.1242/jcs.112.13.2125 PubMed 10362542
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