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Addgene

pCMV5-Flag-EPLIN alpha delta N54
(Plasmid #40934)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5-Flag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EPLIN
  • Species
    H. sapiens (human)
  • Mutation
    deleted amino acids 1-54
  • Entrez Gene
    LIMA1 (a.k.a. EPLIN, LDLCQ8, SREBP3)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Raymond S. Maul

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-Flag-EPLIN alpha delta N54 was a gift from Elizabeth Luna & Scott Maul (Addgene plasmid # 40934 ; http://n2t.net/addgene:40934 ; RRID:Addgene_40934)

  • For your References section:

    Inhibition of anchorage-independent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton. Song Y, Maul RS, Gerbin CS, Chang DD. Mol Biol Cell. 2002 Apr;13(4):1408-16. 10.1091/mbc.01-08-0414 PubMed 11950948
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