pCMV5-Flag-EPLIN beta
(Plasmid #40929)

• Depositing Labs: Elizabeth Luna, Scott Maul
• Publication: Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Backbone

• Vector backbone: pCMV5-Flag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EPLIN beta
• Species: H. sapiens (human)
• Insert Size (bp): 2277
• Entrez Gene: LIMA1 (a.k.a. EPLIN, LDLCQ8, SREBP3)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Raymond S. Maul

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry