pcDNA3-FLAG-KLF5
(Plasmid #40918)

• Depositing Lab: Jin-Tang Dong
• Publication: Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kruppel-like factor 5
• Alt name: KLF5
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• GenBank ID: NM_001730.3
• Entrez Gene: KLF5 (a.k.a. BTEB2, CKLF, IKLF)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (destroyed during cloning)
• 3′ cloning site: EcoR1 (destroyed during cloning)
• 5′ sequencing primer: 5′-GGGGGTACCAT GGCAGAGCTGTGCCCCCTG-3′
• 3′ sequencing primer: 5′-CCCGAATTCCTACTTGGGGGAGCAGATGGAGAGTG-3′

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry