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pGEX-mTAZ-WW (86)
(Plasmid #40915)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40915 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Alt name
    Transcriptional co-Activator with PDZ-binding motif
  • Alt name
    WW domain containing transcription regulator 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    250
  • Mutation
    Contains mTAZ 14-3-3 binding site and WW domain (86 amino acids, 79-164)
  • GenBank ID
    NM_001168281.1 NM_133784.3
  • Entrez Gene
    Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fragments coding for the mTAZ WW domain (amino acids 122-164 and 79-164) were subcloned into pGEX4T2 (Amersham Pharmacia Biotech) for bacterial expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-mTAZ-WW (86) was a gift from Michael Yaffe (Addgene plasmid # 40915 ; http://n2t.net/addgene:40915 ; RRID:Addgene_40915)

  • For your References section:

    TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. Kanai F, Marignani PA, Sarbassova D, Yagi R, Hall RA, Donowitz M, Hisaminato A, Fujiwara T, Ito Y, Cantley LC, Yaffe MB. EMBO J. 2000 Dec 15. 19(24):6778-91. 10.1093/emboj/19.24.6778 PubMed 11118213
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