pcDNA3.1(-)-chicken HA-GPIbb
(Plasmid #40912)

• Depositing Lab: Renhao Li
• Publication: Renhao Lab chicken GPIb-IX plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 6027
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: chicken HA-GPIbb
• Alt name: glycoprotein Ib beta
• Species: G. gallus (chicken)
• Insert Size (bp): 597
• Entrez Gene: GP1BB
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GPIbb insert in the plasmid was cloned from chicken blood plasma.

Please note that Addgene's sequencing results differ from the depositing laboratory's predicted sequence and GenBank's predicted sequence XM_003642172.1. When compared to XP_003642220.1, the HA tag appears to be inserted at aa20. The insertion of the HA tag was based on the signal peptide predictions for GPIbb and the construct has been tested functionally for proper surface expression of the protein by the depositing lab.