pcDNA3.1(-)-chicken Flag-GPIba
(Plasmid #40911)

• Depositing Lab: Renhao Li
• Publication: Renhao Lab chicken GPIb-IX plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 7611
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: chicken Flag-GPIba
• Alt name: platelet glycoprotein Ib alpha
• Species: G. gallus (chicken)
• Insert Size (bp): 2184
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GPIba insert in the plasmid was cloned from chicken blood plasma.

Please note that Addgene's sequencing results differ from the depositing laboratory's predicted sequence and GenBank's predicted sequence XM_003642839. When compared to XP_003642887.1, the FLAG tag appears to be inserted at aa25, aa58-83 appear to be missing along with a few other mutations relative to the predicted reference sequence. The insertion of the FLAG tag was based on the signal peptide predictions for GPIba and the construct has been tested functionally for proper surface expression of the protein by the depositing lab.