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Addgene

pcDNA3-KLF5-his
(Plasmid #40903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6800
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    none
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kruppel-like factor 5
  • Alt name
    KLF5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_001730.3
  • Entrez Gene
    KLF5 (a.k.a. BTEB2, CKLF, IKLF)
  • Promoter CMV
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (destroyed during cloning)
  • 3′ cloning site Not1 (destroyed during cloning)
  • 5′ sequencing primer 5′-TTTGAATTCATGGCTACAAGGGTGC-3′
  • 3′ sequencing primer 5′-TTTGCGGCCGCGTTCTGGTGCCTCTTCATA-3′
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-KLF5-his was a gift from Jin-Tang Dong (Addgene plasmid # 40903 ; http://n2t.net/addgene:40903 ; RRID:Addgene_40903)

  • For your References section:

    Oestrogen causes degradation of KLF5 by inducing the E3 ubiquitin ligase EFP in ER-positive breast cancer cells. Zhao KW, Sikriwal D, Dong X, Guo P, Sun X, Dong JT. Biochem J. 2011 Jul 15;437(2):323-33. 10.1042/BJ20101388 PubMed 21542805
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