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Addgene

pcDNA3-KLF5
(Plasmid #40900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Intrivogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kruppel-like factor 5
  • Alt name
    KLF5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_001730.3
  • Entrez Gene
    KLF5 (a.k.a. BTEB2, CKLF, IKLF)
  • Promoter PCMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-KLF5 was a gift from Jin-Tang Dong (Addgene plasmid # 40900 ; http://n2t.net/addgene:40900 ; RRID:Addgene_40900)

  • For your References section:

    A possible tumor suppressor role of the KLF5 transcription factor in human breast cancer. Chen C, Bhalala HV, Qiao H, Dong JT. Oncogene. 2002 Sep 26;21(43):6567-72. 10.1038/sj.onc.1205817 PubMed 12242654
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