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Addgene

PM-FRB-mRFP-T2A-FKBP-5-ptase
(Plasmid #40896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmRFP-C1
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PM-FRB-mRFP-T2A-FKBP-5-ptase
  • Insert Size (bp)
    2841

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer GTCGTAACAACTCCGCCC
  • 3′ sequencing primer CAT TTT ATG TTT CAG GTT CAG GG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PM-FRB-mRFP-T2A-FKBP-5-ptase was a gift from Peter Varnai (Addgene plasmid # 40896 ; http://n2t.net/addgene:40896 ; RRID:Addgene_40896)

  • For your References section:

    Acute depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate impairs specific steps in endocytosis of the G-protein-coupled receptor. Toth DJ, Toth JT, Gulyas G, Balla A, Balla T, Hunyady L, Varnai P. J Cell Sci. 2012 May 1;125(Pt 9):2185-97. Epub 2012 Feb 22. 10.1242/jcs.097279 PubMed 22357943
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