pSmac-GFP
(Plasmid #40881)

• Depositing Lab: Douglas Green
• Publication: Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Backbone

• Vector backbone: eGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 6193
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Smac-GFP
• Species: H. sapiens (human)
• Insert Size (bp): 1459
• Entrez Gene: DIABLO (a.k.a. DFNA64, SMAC)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: Bam HI (unknown if destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: GFP antisense

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry