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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5523
- Total vector size (bp) 6418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSmac(1-60)mCherry
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SpeciesH. sapiens (human)
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Insert Size (bp)895
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Entrez GeneDIABLO (a.k.a. DFNA64, SMAC)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Smac(1-60)mCherry was a gift from Douglas Green (Addgene plasmid # 40880 ; http://n2t.net/addgene:40880 ; RRID:Addgene_40880) -
For your References section:
Resistance to caspase-independent cell death requires persistence of intact mitochondria. Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, Munoz-Pinedo C, Green DR. Dev Cell. 2010 May 18;18(5):802-13. 10.1016/j.devcel.2010.03.014 PubMed 20493813