L13-Arl13bGFP
(Plasmid #40879)

• Purpose: 3rd generation transfer vector
• Depositing Lab: Tamara Caspary
• Publication: Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.

Backbone

• Vector backbone: pFCGW-N1
• Backbone size w/o insert (bp): 9462
• Total vector size (bp): 10862
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow bacteria overnight at 30 degrees for good DNA yield.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Arl13b
• Species: M. musculus (mouse)
• Insert Size (bp): 1400
• Entrez Gene: Arl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From Larkins et al (2011), this is the Arl13b-GFP expression virus as described in the Materials and Methods and used in the FRAP experiment shown in Figure 4.