pDEST53-Arl13b20
(Plasmid #40876)

• Depositing Lab: Tamara Caspary
• Publication: Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.

Backbone

• Vector backbone: pcDNA-DEST53
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7767
• Total vector size (bp): 8507
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Arl13b
• Species: M. musculus (mouse)
• Insert Size (bp): 740
• Mutation: deleted Arl N-terminal domain; added stop codon
• Entrez Gene: Arl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In Larkins et al (2011), this is plasmid "C-terminal domain GFP tagged" or "C-term-GFP" as referenced in Figure 2. Amino acids 202-427 of Arl13b was Gateway cloned into pcDNA-DEST53.