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pDEST40-Arl13b11
(Plasmid #40873)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40873 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-DEST40
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7100
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arl13b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1410
  • Mutation
    no stop codon
  • Entrez Gene
    Arl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST40.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST40-Arl13b11 was a gift from Tamara Caspary (Addgene plasmid # 40873 ; http://n2t.net/addgene:40873 ; RRID:Addgene_40873)

  • For your References section:

    Arl13b regulates ciliogenesis and the dynamic localization of Shh signaling proteins. Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T. Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5. 10.1091/mbc.E10-12-0994 PubMed 21976698

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