pCMV-EGFP-T2A-ACREB-WPRE
(Plasmid #40867)

• Depositing Lab: Harold Gainer
• Publication: Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2011 Oct 26.

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Backbone

• Vector backbone: pFastBac1
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACREB
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CCTTGCTGTTCTTCTACGGCA
• 3′ sequencing primer: aagtttaacaacaacaattgca

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a A-4779GT and GG5808TT mutations which do not effect plasmid function.