pcDNA3 tdAPX W41F
(Plasmid #40863)

• Purpose: Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).
• Depositing Lab: Alice Ting
• Publication: Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.

Backbone

• Vector backbone: pcDNA3
• Total vector size (bp): 6996
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tandem dimer pea APX W41F
• Species: pea
• Insert Size (bp): 1608
• Mutation: W41F mutation
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVf
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is for expression of tandem dimer pea APX W41F in mammalian cells under the CMV promoter. In this construct, tdAPX is not fused to a signal peptide, so its expression is whole-cell. The insert is not mammalian codon optimized.