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psiCheck-2-3 x mir-34
(Plasmid #40831)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCheck-2
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 6273
  • Vector type
    Insect Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    three sites partially complementary to miR-34
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    mir-34 (a.k.a. Dmel_CR43033, 34, CR33326, CR43033, Dmel\CR43033, Dmel_CR33326, Mir-34, dme-miR-34, dme-mir-34, miR-34)
  • Promoter SV40
  • Tag / Fusion Protein
    • luciferase (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA

S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC

AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC

the triple(3x)-repeated sequence:

5'TGGCAGTGACCTTAGCATCAACAC-3'

3'ACCGTCACTGGAATCGTAGTTGTG-5'

pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCheck-2-3 x mir-34 was a gift from Phillip Zamore (Addgene plasmid # 40831 ; http://n2t.net/addgene:40831 ; RRID:Addgene_40831)

  • For your References section:

    The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL. Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3. 10.1016/j.cub.2011.09.034 PubMed 22055293
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